In vitro culture utilization in Hungarian triticale breeding program

Abstract

The doubled haploid plants can play a key role in applied research to map the agronomically, botanically important traits and acceleration of the breeding process in crop plants. The efficiency of triticale anther culture was tested by two field- and greenhouse grown winter triticale varieties (GK Idus and GK Szemes). The effect of growing conditions and genotype and their interaction were tested on four androgenic parameters (number of embryo-like structures, total, green and albino plantlets). The androgenesis was induced in each treatment of the two tested genotypes. Cultivar GK Szemes produced more embryo-like structures, total and albino plantlets, while the field grown GK Idus produced the most green plantlets/Petri dish. In the experiments of growing conditions, the efficiency of this tested method was higher in anther culture of field grown materials if compare to greenhouse origin materials. Based on this promising data, we can suggest the using of this method in applied research (production of mapping population) and practical breeding.