Comparative characterization of some fungal laccases
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Abstract
Laccase producing fungi were isolated from environmental samples. They were identified on the basis of ITS
(Internal Transcribed Spacer) sequence analysis. The laccase production of the isolates were investigated and
compared with those of other strains deriving from the Szeged Microbiological Collection and from
mushroom producer’s spawn. The enzyme production were examined in various (e.g. basic, mineral and
inducer containing) liquid media. The pH optimum determinations for laccase activities were carried out in
cell free ferment broths, at pH 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7 and 8, applying 25 mM succinate buffer. The
investigations were based on ABTS [2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)] substrate
measurement method. The results showed that under these conditions, among the ascomycetes strains
investigated the best laccase producer was a Rhizoctonia solani isolate (the SzMC 6252J strain). Among the
basidiomycetes tested, the best laccase producer was a Ganoderma isolate (HM3).
(Internal Transcribed Spacer) sequence analysis. The laccase production of the isolates were investigated and
compared with those of other strains deriving from the Szeged Microbiological Collection and from
mushroom producer’s spawn. The enzyme production were examined in various (e.g. basic, mineral and
inducer containing) liquid media. The pH optimum determinations for laccase activities were carried out in
cell free ferment broths, at pH 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7 and 8, applying 25 mM succinate buffer. The
investigations were based on ABTS [2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)] substrate
measurement method. The results showed that under these conditions, among the ascomycetes strains
investigated the best laccase producer was a Rhizoctonia solani isolate (the SzMC 6252J strain). Among the
basidiomycetes tested, the best laccase producer was a Ganoderma isolate (HM3).
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How to Cite
Sajben-Nagy, Enikő, László Manczinger, Biljana Škrbić, N. Đurišić Mladenović, and Csaba Vágvölgyi. 2012. “Comparative Characterization of Some Fungal Laccases”. Review on Agriculture and Rural Development 1 (1. suppl.):413-17. https://analecta.hu/index.php/rard/article/view/13242.
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